ARSA Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31971
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31971-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ARSA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat testis, 2) rat pancreas, 3) rat skeletal muscle, 4) mouse kidney and 5) human MCF7 lysate with ARSA antibody. Expected/observed molecular weight ~54 kDa.
2 / 7
IHC testing of FFPE human breast cancer tissue with ARSA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 7
IHC testing of FFPE rat lung with ARSA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 7
Flow cytometry testing of human HeLa cells with ARSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ARSA antibody.
5 / 7
Flow cytometry testing of human PC-3 cells with ARSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ARSA antibody.
6 / 7
IF/ICC staining of FFPE human U-2 OS cells with ARSA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
IF/ICC staining of FFPE human U-2 OS cells with ARSA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
❮❯

Western blot testing of 1) rat testis, 2) rat pancreas, 3) rat skeletal muscle, 4) mouse kidney and 5) human MCF7 lysate with ARSA antibody. Expected/observed molecular weight ~54 kDa.
IHC testing of FFPE human breast cancer tissue with ARSA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat lung with ARSA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with ARSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ARSA antibody.
Flow cytometry testing of human PC-3 cells with ARSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ARSA antibody.
IF/ICC staining of FFPE human U-2 OS cells with ARSA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of FFPE human U-2 OS cells with ARSA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the ARSA antibody should be determined by the researcher.
Description:
Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QALKQLQLLKAQLDAAVTFGPSQVARGED of human ARSA were used as the immunogen for the ARSA antibody.
Limitation:
This ARSA antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15289