ASIC3 Antibody

Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells

The stated application concentrations are suggested starting amounts. Titration of the ASIC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Acid-Sensing Ion Channel 3, also known as Testis sodium channel (TNAC1) or Dorsal root acid-sensing ion channel (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al.(1998) mapped the gene to chromosome 7q35. De Weille et al.(1998) found that the human protein supported an H(+)-gated cation current in COS cells with kinetics similar to those of the rat protein. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the N-terminus of human Acid-Sensing Ion Channel 3 (FLYQVAERVRYYREFHHQ) was used as the immunogen for this ASIC3 antibody.

This ASIC3 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

Q9UHC3