Ataxin 1 Antibody / ATXN1

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NSJ Bioreagents
Product Code: NSJ-R32138
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32138-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ATXN1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) MCF7, 2) COLO-320 and 3) PANC-1 lysate with ATXN1 antibody. Commonly observed molecular weight: 87-105 kDa.
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IHC testing of FFPE human lung cancer with ATXN1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC staining of FFPE human colon cancer tissue with ATXN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain tissue with ATXN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain tissue with ATXN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human HepG2 cells with ATXN1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with ATXN1 antibody. Commonly observed molecular weight: 87-105 kDa.
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Flow cytometry testing of human SiHa cells with ATXN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN1 antibody.
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Western blot testing of human 1) MCF7, 2) COLO-320 and 3) PANC-1 lysate with ATXN1 antibody. Commonly observed molecular weight: 87-105 kDa.
IHC testing of FFPE human lung cancer with ATXN1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC staining of FFPE human colon cancer tissue with ATXN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with ATXN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with ATXN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HepG2 cells with ATXN1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with ATXN1 antibody. Commonly observed molecular weight: 87-105 kDa.
Flow cytometry testing of human SiHa cells with ATXN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the ATXN1 antibody should be determined by the researcher.
Description:
Ataxin-1 is a protein that in humans is encoded by the ATXN1 gene. The ATXN1 gene had been mapped to 6p23 by in situ hybridization. Ataxin-1 (ATXN1), a causative factor for spinocerebellar ataxia type 1 (SCA1), and the related Brother of ATXN1 (BOAT1) are human proteins involved in transcriptional repression. ATXN1 and BOAT1 might participate in several Notch-controlled developmental and pathological processes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ESRKLEKSEDEPPLTLPKPSLIPQEVKICIE of human Ataxin 1 were used as the immunogen for the ATXN1 antibody.
Limitation:
This ATXN1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P54253