Ataxin 3 Antibody / ATXN3

Contact us
NSJ Bioreagents
Product Code: NSJ-R32137
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32137-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ATXN3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) rat brain, 2) human COLO320 and 3) HeLa lysate with ATXN3 antibody. Predicted/observed molecular weight ~42 kDa.
2 / 5
IHC testing of FFPE human lung cancer with ATXN3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of FFPE human MCF7 cells with ATXN3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Flow cytometry testing of human A549 cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.
5 / 5
Flow cytometry testing of human SiHa cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.
❮❯

Western blot testing of 1) rat brain, 2) human COLO320 and 3) HeLa lysate with ATXN3 antibody. Predicted/observed molecular weight ~42 kDa.
IHC testing of FFPE human lung cancer with ATXN3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Immunofluorescent staining of FFPE human MCF7 cells with ATXN3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.
Flow cytometry testing of human SiHa cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the ATXN3 antibody should be determined by the researcher.
Description:
ATXN3 (Ataxin 3), also known as AT3, MJD GENE, MJD1, SCA3 GENE, ATX3, JOS, Spinocerebellar ataxia-3, Machado-Joseph disease protein 1, is a protein that in humans is encoded by the ATXN3 gene. ATXN3 ranges in size from 360 to 374 amino acids. Using Northern blot analysis showed that ATXN3 mRNA was ubiquitously expressed in human tissues. They detected at least 4 ATXN3 transcripts of 1.4, 1.8, 4.5, and 7.5 kb and suggested that the different mRNA species probably result from differential splicing and polyadenylation. Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by the ATXN3 gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is an inverse correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Ataxin-3 interacted with 2 human homologs of the yeast DNA repair protein RAD23, HHR23A (RAD23A) and HHR23B (RAD23B). Both normal and mutant ataxin-3 proteins interacted with the ubiquitin-like domain at the N terminus of the HHR23 proteins, which is a motif important for nucleotide excision repair. However, in HEK 293 cells, HHR23A was recruited to intranuclear inclusions formed by the mutant ataxin-3 through its interaction with ataxin-3.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids EEDLQRALALSRQEIDMEDEEADLRRAIQ of human Ataxin 3 were used as the immunogen for the ATXN3 antibody.
Limitation:
This ATXN3 antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P54252