ATF2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31552
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31552-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ATF2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of ATF2 antibody and recombinant human protein (0.5ng)
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IHC testing of FFPE human intestinal cancer tissue with ATF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE mouse brain tissue with ATF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE rat brain tissue with ATF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of frozen mouse brain tissue with ATF2 antibody at 1ug/ml.
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IHC testing of frozen rat brain tissue with ATF2 antibody at 1ug/ml.
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Flow cytometry testing of human K562 cells with ATF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATF2 antibody.
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Flow cytometry testing of human HepG2 cells with ATF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATF2 antibody.
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Western blot testing of human 1) HepG2, 2) K562, 3) SH-SY5Y, 4) U-87 MG, 5) A549, 6) MOLT-4 and 7) HEL cell lysate with ATF2 antibody. Expected molecular weight: 65-70 kDa.
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Western blot testing of 1) rat brain, 2) rat thymus, 3) mouse brain and 4) mouse thymus tissue lysate with ATF2 antibody. Expected molecular weight: 65-70 kDa.
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Western blot testing of ATF2 antibody and recombinant human protein (0.5ng)
IHC testing of FFPE human intestinal cancer tissue with ATF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse brain tissue with ATF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat brain tissue with ATF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of frozen mouse brain tissue with ATF2 antibody at 1ug/ml.
IHC testing of frozen rat brain tissue with ATF2 antibody at 1ug/ml.
Flow cytometry testing of human K562 cells with ATF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATF2 antibody.
Flow cytometry testing of human HepG2 cells with ATF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATF2 antibody.
Western blot testing of human 1) HepG2, 2) K562, 3) SH-SY5Y, 4) U-87 MG, 5) A549, 6) MOLT-4 and 7) HEL cell lysate with ATF2 antibody. Expected molecular weight: 65-70 kDa.
Western blot testing of 1) rat brain, 2) rat thymus, 3) mouse brain and 4) mouse thymus tissue lysate with ATF2 antibody. Expected molecular weight: 65-70 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Activating transcription factor 2 is a protein that in humans is encoded by the ATF2 gene. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. It binds to the cAMP-responsive element (CRE), an octameric palindrome. ATF2 forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus, it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
1386
Immunogen:
Human partial recombinant protein (AA 93-450) was used as the immunogen for this ATF2 antibody.
Limitation:
This ATF2 antibody is available for research use only.
Localization:
Nuclear, Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat