ATG7 Antibody

NSJ Bioreagents
Product Code: NSJ-R31385
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31385-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the ATG7 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human HeLa cells with ATG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7 antibody.~
2 / 2
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human K562, 4) hum

Flow cytometry testing of human HeLa cells with ATG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7 antibody.~
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human K562, 4) hum

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, the protein can regulate p53-dependent cell cycle and cell death pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 652-669 (KVLDQYEREGFNFLAKVF-human) were used as the immunogen for this ATG7 antibody.
Limitation:
This ATG7 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95352