ATP5H Antibody

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NSJ Bioreagents
Product Code: NSJ-R31741
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31741-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ATP5H antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human MCF7 cells with ATP5H antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
Immunofluorescent staining of FFPE human T-47D cells with ATP5H antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
IHC staining of FFPE human rectal cancer tissue with ATP5H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U-87 MG, 4) human A549, 5) monkey COS-7, 6) human SW620, 7) human Jurkat and 8) human 293T cell lysate with ATP5H antibody. Expected molecular weight: ~22 kDa.
5 / 5
Western blot testing of 1) rat brain, 2) rat liver, 3) rat heart, 4) rat PC-12, 5) mouse brain, 6) mouse liver, 7) mouse heart and 8) mouse ANA-1 cell lysate with ATP5H antibody. Expected molecular weight: ~22 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with ATP5H antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human T-47D cells with ATP5H antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer tissue with ATP5H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U-87 MG, 4) human A549, 5) monkey COS-7, 6) human SW620, 7) human Jurkat and 8) human 293T cell lysate with ATP5H antibody. Expected molecular weight: ~22 kDa.
Western blot testing of 1) rat brain, 2) rat liver, 3) rat heart, 4) rat PC-12, 5) mouse brain, 6) mouse liver, 7) mouse heart and 8) mouse ANA-1 cell lysate with ATP5H antibody. Expected molecular weight: ~22 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ATP5H antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ATP5H is the 'd' subunit of Mitochondrial ATP synthase complex which catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo has nine known subunits (a, b, c, d, e, f, g, F6 and 8). The ATP5H gene encodes the d subunit of the Fo complex.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
10476
Immunogen:
Human partial recombinant protein (AA 2-161) was used as the immunogen for this ATP5H antibody.
Limitation:
This ATP5H antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat, Monkey