B Raf Antibody (BRAF)

NSJ Bioreagents
Product Code: NSJ-R30946
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30946-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the B Raf antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of B Raf antibody and Lane 1: rat brain; 2: rat testis; 3: rat liver; 4: (h) SW620; 5: (h) COLO320; 6: (r) PC-12 cell lysate

Western blot testing of B Raf antibody and Lane 1: rat brain; 2: rat testis; 3: rat liver; 4: (h) SW620; 5: (h) COLO320; 6: (r) PC-12 cell lysate

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the B Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a serine/threonine-protein kinase called B Raf, also known as RAFB1. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts. B Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 344-362 (QPFRPADEDHRNQFGQRDR) were used as the immunogen for this B Raf antibody (100% homologous in human, mouse and rat).
Limitation:
This B Raf antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15056