BAG5 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32800
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32800-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the BAG5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human T-47D cells with BAG5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human PC-3, 2) human U-87 MG, 3) human HeLa, 4) human K562, 5) rat brain, 6) rat stomach, 7) mouse brain and 8) mouse stomach lysate with BAG5 antibody. Predicted molecular weight: ~51 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with BAG5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BAG5 antibody.
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Immunofluorescent staining of FFPE human T-47D cells with BAG5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human PC-3, 2) human U-87 MG, 3) human HeLa, 4) human K562, 5) rat brain, 6) rat stomach, 7) mouse brain and 8) mouse stomach lysate with BAG5 antibody. Predicted molecular weight: ~51 kDa.
Flow cytometry testing of human HL60 cells with BAG5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BAG5 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the BAG5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. It is mapped to 14q32.33. The protein encoded by this gene is a member of the BAG1-related protein family. Bag5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids N389-Y447 from the human protein were used as the immunogen for the BAG5 antibody.
Limitation:
This BAG5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UL15