Bcl-2 Antibody
| Code | Size | Price |
|---|
| NSJ-R30004-100ug | 100 ug | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: Bcl-2-100, aka BL-2
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
After reconstitution, the Bcl-2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
Documents
Further Information
Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml in formalin/acetone fixed tissues,Immunocytochemistry: Suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Immunoreactive BCL2 protein was detected in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of the protein, regardless of whether the breakpoint was located in or at a distance from the gene. Overexpression blocks the apoptotic death of a pro-B-lymphocyte cell line.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
596
Immunogen:
Amino acids 41-54 of the human protein were used as the immunogen for this Bcl-2 antibody.
Limitation:
This Bcl-2 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human