BDNF Antibody

NSJ Bioreagents
Product Code: NSJ-R31503
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31503-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BDNF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of BDNF antibody (0.5ug/ml) and rat brain lysate
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IHC-P testing of mouse brain tissue
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IHC-P testing of rat brain tissue
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Flow cytometry testing of human U-87 MG cells with BDNF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=BDNF antibody.

Western blot testing of BDNF antibody (0.5ug/ml) and rat brain lysate
IHC-P testing of mouse brain tissue
IHC-P testing of rat brain tissue
Flow cytometry testing of human U-87 MG cells with BDNF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=BDNF antibody.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the BDNF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Brain-derived neurotrophic factor is a secreted protein that, in humans, is encoded by the BDNF gene. It is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. It has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
12064
Immunogen:
Human partial recombinant protein (AA 129-247) was used as the immunogen for this BDNF antibody.
Limitation:
This BDNF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P23560