Beclin 1 Antibody
Code | Size | Price |
---|
NSJ-R30945-100ug | 100 ug | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store the lyophilized Beclin 1 antibody at 40. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.
Images
Documents
Further Information
Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Titration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bellini-1 is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene 6 (ATG6). Beclin 1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. The gene encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed Beclin 1 immunoreactivity in many neurons throughout these regions, as well as in some glial cells. It protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that Beclin 1 reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 222-237 (LDQEEAQYQREYSEFK) were used as the immunogen for this Beclin 1 antibody (100% homologous in human, mouse and rat).
Limitation:
This Beclin 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14457