Beclin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31504
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31504-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Beclin 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Beclin 1 antibody and Lane 1: COLO320; 2: HepG2; 3: PANC; 4: A431; 5: SMMC-7721; 6: Jurkat; 7: SW620; 8: U87 lysate. Expected size: 49KD, Observed size: ~57KD
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IHC-P: Beclin 1 antibody testing of mouse spleen tissue
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IHC-P testing of mouse intestine tissue
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IHC-P testing of rat spleen tissue
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IHC-P testing of rat intestine tissue
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Flow cytometry testing of human HepG2 cells with Beclin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beclin 1 antibody.
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Western blot testing of Beclin 1 antibody and Lane 1: COLO320; 2: HepG2; 3: PANC; 4: A431; 5: SMMC-7721; 6: Jurkat; 7: SW620; 8: U87 lysate. Expected size: 49KD, Observed size: ~57KD
IHC-P: Beclin 1 antibody testing of mouse spleen tissue
IHC-P testing of mouse intestine tissue
IHC-P testing of rat spleen tissue
IHC-P testing of rat intestine tissue
Flow cytometry testing of human HepG2 cells with Beclin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beclin 1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Titration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Beclin 1, also known as also known as ATG6 or VPS30 is a protein that in humans is encoded by the BECN1 gene. Beclin 1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. This gene participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Schizophrenia is associated with low levels of the protein in the hippocampus of the affected which causes diminished autophagy which in turn results in increased neuronal cell death. It has been found that Beclin 1 can promote autophagy in autophagy-defective yeast with a targeted disruption of apg6/vps30, and in human MCF7 breast carcinoma cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
8678
Immunogen:
Human partial recombinant protein (AA 1-354) was used as the immunogen for this Beclin 1 antibody.
Limitation:
This Beclin 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat