Bestrophin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4302
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4302-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Bestrophin 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of human 1) A549, 2) SGC-7901 and 3) U-2 OS cell lysate with Bestrophin 1 antibody at 0.5ug/ml. Predicted molecular weight ~68 kDa.
2 / 3
Flow cytometry testing of human HeLa cells with Bestrophin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Bestrophin 1 antibody.
3 / 3
Flow cytometry testing of human LoVo cells with Bestrophin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Bestrophin 1 antibody.
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Western blot testing of human 1) A549, 2) SGC-7901 and 3) U-2 OS cell lysate with Bestrophin 1 antibody at 0.5ug/ml. Predicted molecular weight ~68 kDa.
Flow cytometry testing of human HeLa cells with Bestrophin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Bestrophin 1 antibody.
Flow cytometry testing of human LoVo cells with Bestrophin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Bestrophin 1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Bestrophin 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Bestrophin-1 (Best1) is a protein that, in humans, is encoded by the BEST1 gene. This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RFIYRLALTEEQQLMFEKLTLYCD were used as the immunogen for the Bestrophin 1 antibody.
Limitation:
This Bestrophin 1 antibody is available for research use only.
Localization:
Cell membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O76090