Beta Catenin Antibody

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NSJ Bioreagents
Product Code: NSJ-R32807
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32807-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the beta Catenin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human placenta, 2) human A431, 3) human SK-OV-3, 4) rat heart and 5) mouse testis lysate with beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
2 / 15
IF/ICC staining of FFPE human A431 cells with beta Catenin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 15
Flow cytometry testing of human A549 cells with beta Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Catenin antibody.
4 / 15
IHC testing of FFPE human breast cancer tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 15
IHC testing of FFPE human prostate cancer tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 15
IHC testing of FFPE human liver cancer tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
7 / 15
IHC testing of FFPE mouse liver tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
8 / 15
IHC testing of FFPE mouse small intestine tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
9 / 15
IHC testing of FFPE rat liver tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
10 / 15
IHC testing of FFPE rat small intestine tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
11 / 15
IHC testing of FFPE rat spleen tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
12 / 15
IHC testing of FFPE rat heart tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
13 / 15
Western blot testing of human 1) placenta, 2) PC-3, 3) U-87 MG, 4) Caco-2, 5) HeLa, 6) A549 and 7) K562 lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
14 / 15
Western blot testing of 1) rat heart, 2) mouse heart, 3) mouse lung and 4) mouse liver lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
15 / 15
Immunofluorescent staining of FFPE human intestine cancer tissue with Beta Catenin antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) human placenta, 2) human A431, 3) human SK-OV-3, 4) rat heart and 5) mouse testis lysate with beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
IF/ICC staining of FFPE human A431 cells with beta Catenin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Flow cytometry testing of human A549 cells with beta Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Catenin antibody.
IHC testing of FFPE human breast cancer tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human prostate cancer tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human liver cancer tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse liver tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse small intestine tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat liver tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat heart tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of human 1) placenta, 2) PC-3, 3) U-87 MG, 4) Caco-2, 5) HeLa, 6) A549 and 7) K562 lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
Western blot testing of 1) rat heart, 2) mouse heart, 3) mouse lung and 4) mouse liver lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
Immunofluorescent staining of FFPE human intestine cancer tissue with Beta Catenin antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (BSA-free formulation available)
Application Note:
Optimal dilution of the beta Catenin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose, 0.025% sodium azide
Description:
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids A2-K233 was used as the immunogen for the beta Catenin antibody.
Limitation:
This beta Catenin antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35222