beta Catenin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30312
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30312-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the pAb can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with beta Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC-P: beta Catenin antibody testing of human breast cancer tissue
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IHC-F: beta Catenin antibody testing of rat intestine tissue
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Western blot testing of beta Catenin antibody and Lane 1: MM453; 2: MCF-7; 3: HeLa cell lysate. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
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Immunofluorescent staining of FFPE human A431 cells with beta Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: beta Catenin antibody testing of human breast cancer tissue
IHC-F: beta Catenin antibody testing of rat intestine tissue
Western blot testing of beta Catenin antibody and Lane 1: MM453; 2: MCF-7; 3: HeLa cell lysate. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Titration of the beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. Beta-catenin binds the cytoplasmic domain of some cadherins. It is an adherens junction protein that plays an important role in various aspects of liver biology.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 764-781 (DGLPPGDSNQLAWFDTDL) were used as the immunogen for this beta Catenin antibody (100% homologous in human, mouse and rat).
Limitation:
This beta Catenin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35222