Bid Antibody

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NSJ Bioreagents
Product Code: NSJ-R31123
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31123-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Bid antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE mouse colon tissue with Bid antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat colon tissue with Bid antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of mouse 1) L929, 2) spleen and 3) RAW264.7 cell lysate. Expected molecular weight ~22 kDa.
4 / 4
Flow cytometry testing of mouse Neuro-2a cells with Bid antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bid antibody.
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IHC staining of FFPE mouse colon tissue with Bid antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with Bid antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) L929, 2) spleen and 3) RAW264.7 cell lysate. Expected molecular weight ~22 kDa.
Flow cytometry testing of mouse Neuro-2a cells with Bid antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bid antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Bid antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bid (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al.(1998) mapped the human BID gene to 22q11. Luo et al.(1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse Bid (NQNLFSYVRNLVRNEMD) was used as the immunogen for this Bid antibody.
Limitation:
This Bid antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P70444