Bid Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R32856
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32856-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Bid antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of mouse brain tissue with Bid antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa.
2 / 4
Western blot testing of mouse 1) L929, 2) spleen and 3) RAW264.7 cell lysate with Bid antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa.
3 / 4
IHC staining of FFPE rat spleen tissue with Bid antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Flow cytometry testing of mouse Neuro-2a cells with Bid antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bid antibody.
❮❯

Western blot testing of mouse brain tissue with Bid antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa.
Western blot testing of mouse 1) L929, 2) spleen and 3) RAW264.7 cell lysate with Bid antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa.
IHC staining of FFPE rat spleen tissue with Bid antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of mouse Neuro-2a cells with Bid antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bid antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Bid antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, the human BID gene is mapped to 22q11. It is reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids M1-D195 was used as the immunogen for the Bid antibody.
Limitation:
This Bid antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P70444