BIK Antibody / BCL2-interacting killer

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NSJ Bioreagents
Product Code: NSJ-R32211
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32211-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BIK antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat spleen, 2) rat stomach, 3) rat intestine, 4) human MCF7, 5) human A549 and 6) human SKOV lysate with BIK antibody. Expected molecular weight ~18 kDa but routinely observed at 18-22 kDa.
2 / 7
IHC testing of FFPE human thyroid cancer tissue with BIK antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 7
IHC testing of FFPE mouse spleen with BIK antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 7
IHC testing of FFPE rat spleen with BIK antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 7
Flow cytometry testing of human A431 cells with BIK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BIK antibody.
6 / 7
Flow cytometry testing of human MCF7 cells with BIK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BIK antibody.
7 / 7
Flow cytometry testing of human THP1 cells with BIK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BIK antibody.
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Western blot testing of 1) rat spleen, 2) rat stomach, 3) rat intestine, 4) human MCF7, 5) human A549 and 6) human SKOV lysate with BIK antibody. Expected molecular weight ~18 kDa but routinely observed at 18-22 kDa.
IHC testing of FFPE human thyroid cancer tissue with BIK antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse spleen with BIK antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat spleen with BIK antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Flow cytometry testing of human A431 cells with BIK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BIK antibody.
Flow cytometry testing of human MCF7 cells with BIK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BIK antibody.
Flow cytometry testing of human THP1 cells with BIK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BIK antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the BIK antibody should be determined by the researcher.
Description:
Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 1-123 of human BIK were used as the immunogen for the BIK antibody.
Limitation:
This BIK antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13323