BiP Antibody GRP78

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NSJ Bioreagents
Product Code: NSJ-R30913
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30913-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BiP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of BiP antibody and Lane 1: rat testis; 2: A549; 3: MCF-7; 4: HeLa cell lysate
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IHC-P: BiP antibody testing of rat intestine tissue
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IHC-P: BiP antibody testing of rat cerebellum tissue
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IHC-P: BiP antibody testing of human lung cancer tissue
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ICC testing of BiP antibody and HeLa cells
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IHC-F testing of BiP antibody and rat intestine tissue
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Western blot testing of BiP antibody and Lane 1: rat testis; 2: A549; 3: MCF-7; 4: HeLa cell lysate
IHC-P: BiP antibody testing of rat intestine tissue
IHC-P: BiP antibody testing of rat cerebellum tissue
IHC-P: BiP antibody testing of human lung cancer tissue
ICC testing of BiP antibody and HeLa cells
IHC-F testing of BiP antibody and rat intestine tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the BiP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD(GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum.BiP is an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome. Shen et al.(2002)concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi. The findings ofShen et al.(2002)demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human GRP78/BiP (EWLESHQDADIEDFK) was used as the immunogen for this BiP antibody (100% homologous in human, mouse and rat).
Limitation:
This BiP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11021