BNIP3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4194
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4194-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the BNIP3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) A375 and 3) A549 cell lysate with BNIP3 antibody at 0.5ug/ml. Expected molecular weight: 19-21 kDa.
2 / 2
Western blot testing of rat 1) brain, 2) kidney, 3) heart, 4) testis and mouse 5) brain, 6) kidney, 7) heart and 8) testis lysate with BNIP3 antibody at 0.5ug/ml. Expected molecular weight: 19-21 kDa.
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Western blot testing of human 1) HeLa, 2) A375 and 3) A549 cell lysate with BNIP3 antibody at 0.5ug/ml. Expected molecular weight: 19-21 kDa.
Western blot testing of rat 1) brain, 2) kidney, 3) heart, 4) testis and mouse 5) brain, 6) kidney, 7) heart and 8) testis lysate with BNIP3 antibody at 0.5ug/ml. Expected molecular weight: 19-21 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the BNIP3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death. Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X(L) heterodimerization and confers pro-apoptotic activity; whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KEFLFKHPKRTATLSMRNTSVMKK were used as the immunogen for the BNIP3 antibody.
Limitation:
This BNIP3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q12983