BOB-1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30579
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30579-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BOB-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of BOB-1 antibody and Lane 1: rat spleen tissue lysate~
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IHC-P: BOB-1 antibody testing of rat tonsil tissue
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Western blot testing of BOB-1 antibody and Lane 1: rat spleen tissue lysate~
IHC-P: BOB-1 antibody testing of rat tonsil tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the BOB-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB-1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse BOB-1 (EEEESNTYELNHTLSVEGF) was used as the immunogen for this BOB-1 antibody.
Limitation:
This BOB-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q64693