Bub3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30729
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30729-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Bub3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Bub3 antibody and Lane 1: HeLa; 2: A549; 3: Jurkat; 4: COLO320 cell lysate
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IHC-P: Bub3 antibody testing of human breast cancer tissue
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IHC-P: Bub3 antibody testing of rat testis tissue
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ICC testing of A549 cells
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ICC testing of HeLa cells
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Western blot testing of Bub3 antibody and Lane 1: HeLa; 2: A549; 3: Jurkat; 4: COLO320 cell lysate
IHC-P: Bub3 antibody testing of human breast cancer tissue
IHC-P: Bub3 antibody testing of rat testis tissue
ICC testing of A549 cells
ICC testing of HeLa cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Bub3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC); though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Bub3 (DHQLKMHDLNTDQEN) was used as the immunogen for this Bub3 antibody (100% homologous in human, mouse and rat).
Limitation:
This Bub3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O43684