Calbindin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30006
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30006-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: CB-955, aka CB-D7
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized Calbindin antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Calbindin antibody and Lane 1: rat brain; 2: rat brain; 3: rat kidney; 4: rat lung tissue lysate
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IHC testing of FFPE human breast cancer tissue with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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IHC testing of FFPE human intestinal cancer tissue with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
4 / 7
IHC testing of FFPE mouse kidney with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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IHC testing of FFPE rat kidney with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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Western blot testing of Calbindin antibody and Lane 1: rat brain; 2: rat brain; 3: mouse brain tissue lysate. Expected molecular weight: 27-28 kDa.
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Immunofluorescent testing of FFPE mouse brain with Calbindin antibody (green) and DAPI (blue). HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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Western blot testing of Calbindin antibody and Lane 1: rat brain; 2: rat brain; 3: rat kidney; 4: rat lung tissue lysate
IHC testing of FFPE human breast cancer tissue with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human intestinal cancer tissue with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse kidney with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat kidney with Calbindin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Western blot testing of Calbindin antibody and Lane 1: rat brain; 2: rat brain; 3: mouse brain tissue lysate. Expected molecular weight: 27-28 kDa.
Immunofluorescent testing of FFPE mouse brain with Calbindin antibody (green) and DAPI (blue). HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Titration of the Calbindin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
793
Immunogen:
Bovine kidney calbindin was used as the immunogen for this Calbindin antibody.
Limitation:
This Calbindin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, mouse, rat, bovine