Calbindin Antibody

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NSJ Bioreagents
Product Code: NSJ-R31476
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31476-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Calbindin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Calbindin antibody (0.5ug/ml) and Lane 1: rat brain; 2: rat kidney lysate; Predicted size: 30KD; Observed size: ~37KD
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IHC-P: Calbindin antibody testing of human breast cancer tissue
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IHC-P: Calbindin antibody testing of mouse kidney tissue
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IHC-P testing of rat kidney tissue
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IHC-P testing of human intestinal cancer tissue
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Flow cytometry testing of human HeLa cells with Calbindin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calbindin antibody.
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Flow cytometry testing of human SiHa cells with Calbindin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calbindin antibody.
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Western blot testing of Calbindin antibody (0.5ug/ml) and Lane 1: rat brain; 2: rat kidney lysate; Predicted size: 30KD; Observed size: ~37KD
IHC-P: Calbindin antibody testing of human breast cancer tissue
IHC-P: Calbindin antibody testing of mouse kidney tissue
IHC-P testing of rat kidney tissue
IHC-P testing of human intestinal cancer tissue
Flow cytometry testing of human HeLa cells with Calbindin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calbindin antibody.
Flow cytometry testing of human SiHa cells with Calbindin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calbindin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the Calbindin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
793
Immunogen:
Human partial recombinant protein (AA 2-175) was used as the immunogen for this Calbindin antibody.
Limitation:
This Calbindin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat