Calcineurin A Antibody

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NSJ Bioreagents
Product Code: NSJ-R30007
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30007-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: CN-A1, aka CC-6
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The Calcineurin A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE mouse brain tissue with Calcineurin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat brain tissue with Calcineurin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with Calcineurin A antibody. Expected molecular weight ~59 kDa.
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IHC staining of FFPE mouse brain tissue with Calcineurin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Calcineurin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with Calcineurin A antibody. Expected molecular weight ~59 kDa.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Calcineurin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Calcineurin, the Ca(2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha(PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase(calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast.
Format :
Ascites
Formulation :
0.1mg/ml with 1% BSA and 0.01% sodium azide
Gene ID #:
5534
Immunogen:
Bovine brain calcineurin was used as the immunogen for this Calcineurin A antibody.
Limitation:
This Calcineurin A antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, rat, bovine