Calpain Antibody

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NSJ Bioreagents
Product Code: NSJ-R31743
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31743-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Calpain antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with Calpain antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC-P: Calpain 1 antibody testing of human breast cancer tissue
3 / 4
Western blot testing of Calpain antibody and Lane 1: rat lung; 2: mouse lung; 3: human A549; 4: (h) COLO320; 5: (h) Jurkat. Predicted/expected size ~82KD
4 / 4
Flow cytometry testing of human PC-3 cells with Calpain antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calpain antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Calpain antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Calpain 1 antibody testing of human breast cancer tissue
Western blot testing of Calpain antibody and Lane 1: rat lung; 2: mouse lung; 3: human A549; 4: (h) COLO320; 5: (h) Jurkat. Predicted/expected size ~82KD
Flow cytometry testing of human PC-3 cells with Calpain antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calpain antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Calpain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Calpain 1 is also known as CAPN, CAPN1 and muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CPN1 gene encodes the catalytic subunit of the ubiquitous enzyme, Calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
823
Immunogen:
Human recombinant protein from the CAPN1 catalytic subunit (AA 396-555) was used as the immunogen for this Calpain antibody.
Limitation:
This Calpain antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat