Calpastatin Antibody

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NSJ Bioreagents
Product Code: NSJ-R32585
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32585-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Calpastatin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with Calpastatin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC testing of FFPE human placenta tissue with Calpastatin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 7
IHC testing of FFPE human intestinal cancer tissue with Calpastatin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 7
Flow cytometry testing of human A549 cells with Calpastatin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calpastatin antibody.
5 / 7
Flow cytometry testing of human A431 cells with Calpastatin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calpastatin antibody.
6 / 7
Flow cytometry testing of human U-2 OS cells with Calpastatin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calpastatin antibody.
7 / 7
Western blot testing of human COLO320 cell lysate with Calpastatin antibody at 0.5ug/ml. Predicted molecular weight: 76 kDa but routinely observed at ~110 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with Calpastatin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placenta tissue with Calpastatin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal cancer tissue with Calpastatin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with Calpastatin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calpastatin antibody.
Flow cytometry testing of human A431 cells with Calpastatin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calpastatin antibody.
Flow cytometry testing of human U-2 OS cells with Calpastatin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Calpastatin antibody.
Western blot testing of human COLO320 cell lysate with Calpastatin antibody at 0.5ug/ml. Predicted molecular weight: 76 kDa but routinely observed at ~110 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the Calpastatin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 275-310 (QEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLR-human) were used as the immunogen for the Calpastatin antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P20810