Calretinin Antibody

NSJ Bioreagents
Product Code: NSJ-R30185
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30185-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Calretinin antibody at -200.

Images

1 / 5
Immunofluorescent staining of FFPE mouse brain tissue with Calretinin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE mouse brain tissue with Calretinin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain tissue with Calretinin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC-P: Calretinin antibody testing of human rectal cancer tissue
5 / 5
Western blot testing of Calretinin antibody and HeLa cell lysate. Expected/observed size ~29KD

Immunofluorescent staining of FFPE mouse brain tissue with Calretinin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Calretinin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Calretinin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC-P: Calretinin antibody testing of human rectal cancer tissue
Western blot testing of Calretinin antibody and HeLa cell lysate. Expected/observed size ~29KD

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Calretinin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Calretinin (PQQQPPYLHLAELTA) was used as the immunogen for this Calretinin antibody.
Limitation:
This Calretinin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P22676