Carbonic Anhydrase I Antibody / CA1

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NSJ Bioreagents
Product Code: NSJ-R31859
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31859-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Carbonic Anhydrase I antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human Caco-2 cells with Carbonic Anhydrase I antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human colon adenocarcinoma tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human spleen tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse colon tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat colon tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) human HEL, 2) rat spleen and 3) mouse spleen lysate with Carbonic Anhydrase I antibody. Predicted molecular weight: ~29 kDa.
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Flow cytometry testing of human HEL cells with Carbonic Anhydrase I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carbonic Anhydrase I antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with Carbonic Anhydrase I antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human colon adenocarcinoma tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HEL, 2) rat spleen and 3) mouse spleen lysate with Carbonic Anhydrase I antibody. Predicted molecular weight: ~29 kDa.
Flow cytometry testing of human HEL cells with Carbonic Anhydrase I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carbonic Anhydrase I antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the Carbonic Anhydrase I antibody should be determined by the researcher.
Description:
Carbonic anhydrase I is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 9-261 of human CA1 were used as the immunogen for the Carbonic Anhydrase I antibody.
Limitation:
This Carbonic Anhydrase I antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P00915