CARS Antibody / Cysteinyl-tRNA synthetase

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NSJ Bioreagents
Product Code: NSJ-RQ4008
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4008-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the CARS antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human placental tissue with CARS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with CARS antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
Western blot testing of human 1) HeLa, 2) COLO320, 3) A549, 4) PANC-1 and 5) 22RV1 cell lysate with CARS antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa.
4 / 4
Flow cytometry testing of human A431 cells with CARS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CARS antibody.
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IHC staining of FFPE human placental tissue with CARS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with CARS antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) COLO320, 3) A549, 4) PANC-1 and 5) 22RV1 cell lysate with CARS antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa.
Flow cytometry testing of human A431 cells with CARS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CARS antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CARS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
This CARS gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids D510-Q748 was used as the immunogen for the CARS antibody.
Limitation:
This CARS antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P49589