Caspase 4 Antibody / CASP4 (large subunit)

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NSJ Bioreagents
Product Code: NSJ-RQ4340
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4340-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the Caspase 4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of rat 1) liver, 2) testis, 3) stomach, 4) thymus and human 5) COLO-320, 6) HepG2, 7) 22RV1, 8) PANC-1 and 9) SGC-7901 lysate with Caspase 4 antibody at 0.5ug/ml. Expected molecular weight ~43 kDa (precursor), ~30 kDa (pro domain + large subunit), ~20 kDa (large subunit).
2 / 2
Western blot testing of mouse 1) liver, 2) testis, 3) thymus, 4) lung, 5) HEPA1-6, 6) NIH3T3 and 7) SP20 lysate with Caspase 4 antibody at 0.5ug/ml. Expected molecular weight ~43 kDa (precursor), ~30 kDa (pro domain + large subunit), ~20 kDa (large subunit).
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Western blot testing of rat 1) liver, 2) testis, 3) stomach, 4) thymus and human 5) COLO-320, 6) HepG2, 7) 22RV1, 8) PANC-1 and 9) SGC-7901 lysate with Caspase 4 antibody at 0.5ug/ml. Expected molecular weight ~43 kDa (precursor), ~30 kDa (pro domain + large subunit), ~20 kDa (large subunit).
Western blot testing of mouse 1) liver, 2) testis, 3) thymus, 4) lung, 5) HEPA1-6, 6) NIH3T3 and 7) SP20 lysate with Caspase 4 antibody at 0.5ug/ml. Expected molecular weight ~43 kDa (precursor), ~30 kDa (pro domain + large subunit), ~20 kDa (large subunit).

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Further Information

Application Details :
Western blot: 0.5-1ug/ml, Direct ELISA: 0.1-0.5ug/ml (mouse recombinant protein)
Application Note:
Optimal dilution of the Caspase 4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids E108-T195 was used as the immunogen for the Caspase 4 antibody.
Limitation:
This Caspase 4 antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P70343