Caspase-1 Antibody (p10)

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml

The stated application concentrations are suggested starting points. Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Caspase-1, an apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL-18, and IL-33 precursor proteins. It was purified from the cytosol of the THP monocytic cell line and found that the active protease was made up of two peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The CASP1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal metaphase chromosomes. Caspase-1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Amino acids 249-262 (VFIGRLIEHMQEYA) from the large subunit were used as the immunogen for this Caspase-1 antibody.

This Caspase-1 antibody is available for research use only.

Antigen affinity

Human

P29466