Caspase-3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30947
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30947-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Caspase-3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Caspase-3 antibody (0.5ug/ml) and Lane 1: rat liver; 2: MCF-7; 3: SMMC-7721; 4: HT1080 lysate~
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Western blot testing of 1) rat heart, 2) rat liver, 3) rat thymus, 4) MCF7, 5) SMMC and 6) HT1080 lysate with Caspase-3 antibody
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Western blot testing of Caspase-3 antibody (0.5ug/ml) and Lane 1: rat liver; 2: MCF-7; 3: SMMC-7721; 4: HT1080 lysate~
Western blot testing of 1) rat heart, 2) rat liver, 3) rat thymus, 4) MCF7, 5) SMMC and 6) HT1080 lysate with Caspase-3 antibody

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cysteine-aspartic acid protease 3, also known as Apopain, CPP32, CPP32B, and YAMA, is a member of the cysteine-aspartic acid protease family. Western blot analysis of three cell lines revealed the prominent CASP3 band at 32 kD and 20 kD. Several human cancer cell lines showed coexpression of both variants at the mRNA and protein levels. Overexpression of the catalytically inactive Caspase-3 by human kidney cells offered some resistance to inducers of apoptosis, and CASP3 accumulation could be enhanced with addition of proteasome inhibitors. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Alternative splicing of this gene results in two transcript variants that encode the same protein. Orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Nicholson et al. developed a potent peptide aldehyde inhibitor and showed that it prevented apoptotic events in vitro, suggesting that the protein is important for the initiation of apoptotic cell death.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 207-220 (RNSKDGSWFIQSLC-human) were used as the immunogen for this Caspase-3 antibody (100% mouse homology). This amino acid sequence is from the small subunit.
Limitation:
This Caspase-3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P42574