Caspase-3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31602
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31602-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Caspase-3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Caspase-3 antibody and Lane 1: rat liver; 2: rat thymus; 3: SMMC-7721 lysate. Predicted molecular weight ~32 kDa.
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IHC-P: Caspase-3 antibody testing of human lung cancer tissue
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IHC-P: Caspase-3 antibody testing of mouse intestine tissue
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IHC-P: Caspase-3 antibody testing of rat intestine tissue
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Western blot testing of Caspase-3 antibody and recombinant human protein (0.5ng)
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Flow cytometry testing of human K562 cells with Caspase-3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Caspase-3 antibody.
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Western blot testing of Caspase-3 antibody and Lane 1: rat liver; 2: rat thymus; 3: SMMC-7721 lysate. Predicted molecular weight ~32 kDa.
IHC-P: Caspase-3 antibody testing of human lung cancer tissue
IHC-P: Caspase-3 antibody testing of mouse intestine tissue
IHC-P: Caspase-3 antibody testing of rat intestine tissue
Western blot testing of Caspase-3 antibody and recombinant human protein (0.5ng)
Flow cytometry testing of human K562 cells with Caspase-3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Caspase-3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caspase-3 is an enzyme which interacts with Survivin, XIAP, CFLAR, CASP8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. They exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. CASP3 is the predominant cysteine-aspartic acid protease involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. And protein activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the proteins influence on bone mineral density should be considered in any in vivo application of Caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
836
Immunogen:
Human partial recombinant protein (AA 67-175) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the large subunit.
Limitation:
This Caspase-3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat