Caspase-8 Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting points. Titration of the Caspase-8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Caspase-8 is also known as CASP8, CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

12370

Mouse partial recombinant protein (AA 388-480) was used as the immunogen for this Caspase-8 antibody. This sequence is from the small subunit.

This Caspase-8 antibody is available for research use only.

Antigen affinity

Mouse