Caspase-9 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30673
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30673-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Caspase-9 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Caspase-9 antibody and Lane 1: SMMC-7721; 2: MCF-7; 3: CEM; 4: Jurkat; 5: Raji; 6: HeLa~
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IHC-P: Caspase-9 antibody testing of human tonsil tissue
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Western blot testing of Caspase-9 antibody and Lane 1: SMMC-7721; 2: MCF-7; 3: CEM; 4: Jurkat; 5: Raji; 6: HeLa~
IHC-P: Caspase-9 antibody testing of human tonsil tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Caspase-9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caspase-9, also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in CASP3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by Cytochrome C and dATP. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of Cytochrome C and dATP, an event that leads to CASP9 activation. Activity increases dramatically upon association with the apoptosome complex. And the majority of CASP9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 183-198 (SNIDCEKLRRRFSSLH-human) were used as the immunogen for this Caspase-9 antibody. This sequence is on the large subunit.
Limitation:
This Caspase-9 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P55211