Cathepsin D Antibody

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NSJ Bioreagents
Product Code: NSJ-R31904
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31904-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cathepsin D antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human MCF7 cells with Cathepsin D antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC testing of FFPE human intestinal cancer wtih Cathepsin D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) HepG2, 2) A549 and 3) PANC cell lysate with Cathepsin D antibody. Expected molecular weight: 43-46 kDa, 28 kDa (heavy chain), 15 kDa (light chain).
4 / 4
Flow cytometry testing of human SiHa cells with Cathepsin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cathepsin D antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with Cathepsin D antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal cancer wtih Cathepsin D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) HepG2, 2) A549 and 3) PANC cell lysate with Cathepsin D antibody. Expected molecular weight: 43-46 kDa, 28 kDa (heavy chain), 15 kDa (light chain).
Flow cytometry testing of human SiHa cells with Cathepsin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cathepsin D antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Cathepsin D antibody should be determined by the researcher.
Description:
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 201-412 of human Cathepsin D were used as the immunogen for the Cathepsin D antibody.
Limitation:
This Cathepsin D antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P07339