Caveolin-2 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31584
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31584-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized Caveolin-2 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE rectal cancer with Caveolin-2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 7
Immunofluorescent staining of FFPE human A549 cells with Caveolin-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 7
IHC staining of FFPE human rectal cancer with Caveolin-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat lung with Caveolin-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Western blot testing testing of human 1) HT1080, 2) U-2 OS, 3) Caco-2, 4) HeLa, 5) A549, 6) rat lung and 7) mouse lung lysate with Caveolin-2 antibody. Predicted molecular weight ~18 kDa.
6 / 7
Western blot testing of Caveolin-2 antibody and recombinant human protein (0.5ng).
7 / 7
Flow cytometry testing of human A549 cells with Caveolin-2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caveolin-2 antibody.
❮❯

Immunofluorescent staining of FFPE rectal cancer with Caveolin-2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human A549 cells with Caveolin-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer with Caveolin-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung with Caveolin-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing testing of human 1) HT1080, 2) U-2 OS, 3) Caco-2, 4) HeLa, 5) A549, 6) rat lung and 7) mouse lung lysate with Caveolin-2 antibody. Predicted molecular weight ~18 kDa.
Western blot testing of Caveolin-2 antibody and recombinant human protein (0.5ng).
Flow cytometry testing of human A549 cells with Caveolin-2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caveolin-2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunofluorescence: 2-4ug/ml
Application Note:
Titration of the Caveolin-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caveolin-2 is a protein that in humans is encoded by the CAV2 gene. It is mapped to 7q31.1-q31.2. The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. Caveolin-2 is a protein related to caveolin-1 which is derived caveolin-enriched membranes. CAV2 and CAV1 are similar in most respects and they differ in their functional interactions with heterotrimeric G proteins. Both of them are expressed in neuronal cells. CAV2 was upregulated in response to neuronal cell injury.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
858
Immunogen:
Human partial recombinant protein (AA 1-162) was used as the immunogen for this Caveolin-2 antibody.
Limitation:
This Caveolin-2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat