CD11b Antibody / MAC-1 / ITGAM

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NSJ Bioreagents
Product Code: NSJ-RQ4351
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4351-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CD11b antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen and 4) mouse thymus lysate with CD11b antibody at 0.5ug/ml. Expected molecular weight: ~95 kDa and ~170 kDa.
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Flow cytometry testing of human HL-60 cells with CD11b antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD11b antibody.
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Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen and 4) mouse thymus lysate with CD11b antibody at 0.5ug/ml. Expected molecular weight: ~95 kDa and ~170 kDa.
Flow cytometry testing of human HL-60 cells with CD11b antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD11b antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD11b, or Macrophage-1 alpha (MAC-1), is a glycoprotein encoded by the ITGAM gene. It has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids N129-E336 was used as the immunogen for the CD11b antibody.
Limitation:
This CD11b antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11215