CD14 Antibody (Monocyte and Macrophage Marker)

Contact us
NSJ Bioreagents
Product Code: NSJ-R32821
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32821-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD14 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC testing of FFPE mouse spleen tissue with CD14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 5
IHC testing of FFPE rat spleen tissue with CD14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 5
IHC testing of FFPE rat lymph tissue with CD14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 5
Western blot testing of 1) mouse thymus and 2) mouse spleen with CD14 antibody at 0.5ug/ml. Predicted molecular weight ~40 kDa (unmodified) and 50-55 kDa (glycosylated).
5 / 5
Flow cytometry testing of mouse PBM cells with Cd14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd14 antibody.
❮❯

IHC testing of FFPE mouse spleen tissue with CD14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with CD14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lymph tissue with CD14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) mouse thymus and 2) mouse spleen with CD14 antibody at 0.5ug/ml. Predicted molecular weight ~40 kDa (unmodified) and 50-55 kDa (glycosylated).
Flow cytometry testing of mouse PBM cells with Cd14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd14 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,ELISA (Capture, recombinant mouse protein): 0.1-0.5ug/ml (BSA-free formulation available)
Application Note:
Optimal dilution of the CD14 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55 kDa glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids S16-A260 was used as the immunogen for the CD14 antibody.
Limitation:
This CD14 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P10810