CD163 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4259
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4259-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD163 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human spleen tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
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IHC staining of FFPE human placental tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
3 / 8
IHC staining of FFPE human liver cancer tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
4 / 8
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
5 / 8
IHC staining of FFPE human appendiceal adenocarcinoma tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
6 / 8
IHC staining of FFPE human lung cancer tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
7 / 8
IHC staining of FFPE human rectal cancer tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
8 / 8
Western blot testing of human 1) HCCT and 2) HCCP cell lysate with CD163 antibody. Expected molecular weight: 125-175 kDa depending on glycosylation level.
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IHC staining of FFPE human spleen tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human placental tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human liver cancer tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human lung cancer tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human rectal cancer tissue with CD163 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HCCT and 2) HCCP cell lysate with CD163 antibody. Expected molecular weight: 125-175 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CD163 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids T47-E201 was used as the immunogen for the CD163 antibody.
Limitation:
This CD163 antibody is available for research use only.
Localization:
Cytoplasm, cell membrane, secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q86VB7