CD18 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30705
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30705-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD18 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of CD18 antibody and Lane 1: Jurkat cell lysate ; 2: CEM; 3: HT1080; 4: SMMC-7721; 5: HeLa cell lysate. Predicted/Observed size: 85~95KD depending on glycosylation level~
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IHC-P: CD18 antibody testing of human breast cancer tis
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Western blot testing of CD18 antibody and Lane 1: Jurkat cell lysate ; 2: CEM; 3: HT1080; 4: SMMC-7721; 5: HeLa cell lysate. Predicted/Observed size: 85~95KD depending on glycosylation level~
IHC-P: CD18 antibody testing of human breast cancer tis

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ITGB2 (Integrin beta 2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2/CD18 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans, lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the inside-out signaling pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human CD18 (LREYRRFEKEKLKSQWN) was used as the immunogen for this CD18 antibody.
Limitation:
This CD18 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05107