CD23 Antibody

Product Code: NSJ-R31482

Code	Size	Price

NSJ-R31482-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Immunohistochemistry- Frozen Section (IHC-F)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the CD23 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10

Western blot testing of CD23 antibody and mouse liver lysate. Expected size 37~45KD

2 / 10

IHC-P: CD23 antibody testing of human tonsil tissue

3 / 10

IHC-P: CD23 antibody testing of mouse spleen tissue

4 / 10

IHC-P: CD23 antibody testing of rat spleen tissue

5 / 10

Flow cytometry testing of human PBMs with CD23 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD23 antibody.

6 / 10

Flow cytometry testing of mouse PBMs with CD23 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD23 antibody.

7 / 10

Immunofluorescent staining of FFPE mouse lymph tissue with CD23 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

8 / 10

Immunofluorescent staining of FFPE rat lymph tissue with CD23 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

9 / 10

Western blot testing of CD23 antibody and 1) rat spleen and 2) rat thymus lysate. Expected molecular weight: 37~45 kDa.

10 / 10

IHC staining of frozen mouse spleen tissue with CD23 antibody.

❮❯

Documents

Further Information

Application Details :

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

The stated application concentrations are suggested starting amounts. Titration of the CD23 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Description:

CD23, also known as Fc epsilon RII, or FceRII, is the low-affinity receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Gene ID #:

14128

Immunogen:

Mouse partial recombinant protein (AA 50-331) was used as the immunogen for this CD23 antibody.

Limitation:

This CD23 antibody is available for research use only.

Purity:

Antigen affinity

Species Reactivity :

Human, Mouse, Rat