CD24 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4392
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4392-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the CD24 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human K562 cells with CD24 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) rat smooth muscle, 2) rat stomach, 3) mouse smooth muscle, 4) mouse stomach and 5) mouse RAW246.7 lysate with CD24 antibody at 0.5ug/ml. Expected molecular weight: 20-70 kDa depending on glycosylation level.
3 / 4
Flow cytometry testing of human Jurkat cells with CD24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD24 antibody.
4 / 4
Western blot testing of human 1) HeLa, 2) placenta, 3) K562, 4) A549 and 5) Jurkat lysate with CD24 antibody at 0.5ug/ml. Expected molecular weight: 20-70 kDa depending on glycosylation level.
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Immunofluorescent staining of FFPE human K562 cells with CD24 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat smooth muscle, 2) rat stomach, 3) mouse smooth muscle, 4) mouse stomach and 5) mouse RAW246.7 lysate with CD24 antibody at 0.5ug/ml. Expected molecular weight: 20-70 kDa depending on glycosylation level.
Flow cytometry testing of human Jurkat cells with CD24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD24 antibody.
Western blot testing of human 1) HeLa, 2) placenta, 3) K562, 4) A549 and 5) Jurkat lysate with CD24 antibody at 0.5ug/ml. Expected molecular weight: 20-70 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the CD24 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Signal transducer CD24, also known as cluster of differentiation 24 or heat stable antigen CD24 (HSA), is a protein that in humans is encoded by the CD24 gene. This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids SETTTGTSSNSSQSTSNTGLAPNPTNATTKAA from the human protein were used as the immunogen for the CD24 antibody.
Limitation:
This CD24 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P25063