Cd252 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4110
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4110-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cd252 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 2
IHC testing of FFPE rat small intestine tissue with Cd252 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 2
Western blot testing of 1ng of recombinant mouse protein with Cd252 antibody at 0.5ug/ml. Predicted molecular weight: 22-35 kDa depending on glycosylation level.
❮❯

IHC testing of FFPE rat small intestine tissue with Cd252 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1ng of recombinant mouse protein with Cd252 antibody at 0.5ug/ml. Predicted molecular weight: 22-35 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,ELISA (Capture): 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cd252 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
OX40L (TNFSF4, CD252) is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse partial protein corresponding to amino acids Q49-L198 was used as the immunogen for the Cd252 antibody.
Limitation:
This Cd252 antibody is available for research use only.
Localization:
Membrane, cytoplasm
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P43488