CD2AP Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4204
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4204-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CD2AP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) COLO320, 5) 22RV1 6) SK-OV-3, 7) rat stomach, 8) rat liver and 9) mouse liver lysate wtih CD2AP antibody at 0.5ug/ml. Predicted molecular weight: 71-80 kDa.
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Flow cytometry testing of human K562 cells with CD2AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
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Flow cytometry testing of human Caco-2 cells with CD2AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
4 / 8
Flow cytometry testing of human A431 cells with CD2AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
5 / 8
IHC staining of FFPE human intestinal cancer with CD2AP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE rat kidney with CD2AP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE mouse kidney with CD2AP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human K562 cells with CD2AP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) COLO320, 5) 22RV1 6) SK-OV-3, 7) rat stomach, 8) rat liver and 9) mouse liver lysate wtih CD2AP antibody at 0.5ug/ml. Predicted molecular weight: 71-80 kDa.
Flow cytometry testing of human K562 cells with CD2AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
Flow cytometry testing of human Caco-2 cells with CD2AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
Flow cytometry testing of human A431 cells with CD2AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
IHC staining of FFPE human intestinal cancer with CD2AP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with CD2AP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney with CD2AP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human K562 cells with CD2AP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CD2AP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids K253-K337 was used as the immunogen for the CD2AP antibody.
Limitation:
This CD2AP antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9Y5K6