CD3 epsilon Antibody

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NSJ Bioreagents
Product Code: NSJ-R31519
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31519-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD3 epsilon antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of CD3 epsilon antibody and Lane 1: Jurkat; 2: CEM lysate. Expected/observed size ~23KD
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Western blot testing of CD3 epsilon antibody and recombinant human protein (0.5ng)
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IHC-P: CD3 epsilon antibody testing of human tonsil tissue
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IHC-P: CD3 epsilon antibody testing of mouse spleen tissue
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IHC-P staining of rat spleen tissue
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IHC-F staining of rat spleen tissue
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IHC-F staining of mouse spleen tissue
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Immunofluorescent staining of FFPE human tonsil tissue with CD3 epsilon antibody (pink) and CD68 mAb (green) at 1ug/ml, and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human tonsil tissue with CD3 epsilon antibody (pink) and CD20 mAb (green) at 2ug/ml, and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Western blot testing of CD3 epsilon antibody and Lane 1: Jurkat; 2: CEM lysate. Expected/observed size ~23KD
Western blot testing of CD3 epsilon antibody and recombinant human protein (0.5ng)
IHC-P: CD3 epsilon antibody testing of human tonsil tissue
IHC-P: CD3 epsilon antibody testing of mouse spleen tissue
IHC-P staining of rat spleen tissue
IHC-F staining of rat spleen tissue
IHC-F staining of mouse spleen tissue
Immunofluorescent staining of FFPE human tonsil tissue with CD3 epsilon antibody (pink) and CD68 mAb (green) at 1ug/ml, and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human tonsil tissue with CD3 epsilon antibody (pink) and CD20 mAb (green) at 2ug/ml, and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD3 epsilon antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD3 epsilon is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. Together with CD3gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, CD3e forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the e, g and d polypeptides are located in the same cluster on chromosome 11. The e-polypeptide plays an essential role in T-cell development.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
916
Immunogen:
Human partial recombinant protein (AA 23-207) was used as the immunogen for this CD3 epsilon antibody.
Limitation:
This CD3 epsilon antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat