CD34 Antibody

Product Code: NSJ-R30399

Code	Size	Price

NSJ-R30399-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Immunohistochemistry- Frozen Section (IHC-F)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the CD34 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of CD34 antibody and Lane 1: Recombinant human CD34 protein 10ng; 2: 5ng; 3: 2.5ng

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IHC-P: CD34 antibody testing of rat kidney

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IHC-P: CD34 antibody testing of human lung cancer

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IHC-F testing of CD34 antibody and rat placenta

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Flow cytometry testing of human A431 cells with CD34 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD34 antibody.

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IHC-P: CD34 antibody testing of human placental tissue with CD34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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IHC-P: CD34 antibody testing of human liver cancer tissue with CD34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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IHC-P: CD34 antibody testing of mouse kidney tissue with CD34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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Documents

Further Information

Application Details :

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

The stated application concentrations are suggested starting amounts. Titration of the CD34 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Description:

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

An amino acid sequence from the C-terminus of human CD34 (QATSRNGHSARQHVVAD) was used as the immunogen for this CD34 antibody (100% homologous human, mouse and rat).

Limitation:

This CD34 antibody is available for research use only.

Purity:

Antigen affinity

Species Reactivity :

Human, Mouse, Rat

Uniprot #:

P28906