CD4 Antibody

NSJ Bioreagents
Product Code: NSJ-R30015
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30015-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: CA-4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Frozen Section (IHC-F)
Storage:
After reconstitution, the CD4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human PBMC with CD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD4 antibody.

Flow cytometry testing of human PBMC with CD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD4 antibody.

Further Information

Application Details :
Immunohistochemistry (Frozen): 1ug/ml-formalin or acetone fixed tissues,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
920
Immunogen:
CD4-transfected mouse T-cell hybridoma (3DT) followed by CD4+ human T-cell CEM cells was used as the immunogen for this CD4 antibody.
Limitation:
This CD4 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human